5a6 anti tau Search Results


5a6  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank 5a6
Figure 1. Eye degeneration phenotypes caused by over-expression of human tau V337M and analysis of the phosphorylation status of human tau V337M in Drosophila (A–C) SEM images of transgenic flies expressing h-tauV337M under the control of the eye-specific promoter element GMR-Gal4 (B, female; C, male) and control flies bearing only the GMR-Gal4 transgene (A). Flies over-expressing h-tauV337M developed rough eye phenotype with disorganized omma- tidial structure, while flies bearing GMR-Gal4 alone showed normal external eye morphology. (D) The phosphorylation status of h-tauV337M was determined by western blot analysis using phosphorylation-dependent antibodies. The h-tauV337M levels were determined using a mix including T14 and T46, or Tau5 and <t>5A6,</t> phosphorylation-independent anti-tau antibodies reacting with total h-tau. The AT8, AD2 and PHF1 antibodies recognize phosphorylated h-tau proteins, whereas Tau-1 recognizes non-phosphorylated h-tau proteins. Actin was used as loading control. (E) Western blot analysis of h-tauWT and h-tauV337M expression in trans- genic flies. Actin was used as loading control.
5a6, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Eye degeneration phenotypes caused by over-expression of human tau V337M and analysis of the phosphorylation status of human tau V337M in Drosophila (A–C) SEM images of transgenic flies expressing h-tauV337M under the control of the eye-specific promoter element GMR-Gal4 (B, female; C, male) and control flies bearing only the GMR-Gal4 transgene (A). Flies over-expressing h-tauV337M developed rough eye phenotype with disorganized omma- tidial structure, while flies bearing GMR-Gal4 alone showed normal external eye morphology. (D) The phosphorylation status of h-tauV337M was determined by western blot analysis using phosphorylation-dependent antibodies. The h-tauV337M levels were determined using a mix including T14 and T46, or Tau5 and 5A6, phosphorylation-independent anti-tau antibodies reacting with total h-tau. The AT8, AD2 and PHF1 antibodies recognize phosphorylated h-tau proteins, whereas Tau-1 recognizes non-phosphorylated h-tau proteins. Actin was used as loading control. (E) Western blot analysis of h-tauWT and h-tauV337M expression in trans- genic flies. Actin was used as loading control.

Journal: Human molecular genetics

Article Title: Cytoskeleton proteins are modulators of mutant tau-induced neurodegeneration in Drosophila.

doi: 10.1093/hmg/ddm011

Figure Lengend Snippet: Figure 1. Eye degeneration phenotypes caused by over-expression of human tau V337M and analysis of the phosphorylation status of human tau V337M in Drosophila (A–C) SEM images of transgenic flies expressing h-tauV337M under the control of the eye-specific promoter element GMR-Gal4 (B, female; C, male) and control flies bearing only the GMR-Gal4 transgene (A). Flies over-expressing h-tauV337M developed rough eye phenotype with disorganized omma- tidial structure, while flies bearing GMR-Gal4 alone showed normal external eye morphology. (D) The phosphorylation status of h-tauV337M was determined by western blot analysis using phosphorylation-dependent antibodies. The h-tauV337M levels were determined using a mix including T14 and T46, or Tau5 and 5A6, phosphorylation-independent anti-tau antibodies reacting with total h-tau. The AT8, AD2 and PHF1 antibodies recognize phosphorylated h-tau proteins, whereas Tau-1 recognizes non-phosphorylated h-tau proteins. Actin was used as loading control. (E) Western blot analysis of h-tauWT and h-tauV337M expression in trans- genic flies. Actin was used as loading control.

Article Snippet: The following anti-human tau antibodies were used in this study: T14 (Zymed Laboratories; South San Francisco, CA, USA; amino acids 83–120, diluted 1:3000) T46 (Zymed Laboratories; amino acids 395–432, diluted 1:3000), Tau5 (Chemicon, Temecula, CA, USA; amino acids 210–230, diluted 1:1000) and 5A6 (Developmental Studies Hybridoma Bank, Ames, IA, USA; amino acids 19–46, diluted 1:6000) which are phosphorylation-independent antibodies that recognize total tau, AT8 (Innogenetics, Gand, Belgium; diluted 1:500) which specifically detect tau phosphorylated at epitopes serine 202 and threonine 205, AD2 (Dr André Delacourte; diluted 1:1000) and PHF1 (Dr Peter Davies; diluted 1:200) which specifically detect tau-phosphorylated at serine 396 and serine 404 and Tau-1 (Dr Lester Binder, diluted 1:2000) which specifically detect tau-phosphorylated dephosphorylated at serines 195, 198, 199 and 202.

Techniques: Over Expression, Phospho-proteomics, Transgenic Assay, Expressing, Control, Western Blot